rheb1 (R&D Systems)
Structured Review
Rheb1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rheb1/Human%2FMouse%2FRat+Rheb+Antibody/10__1523_slash_JNEUROSCI__0449___21__2022_ascii32_-78-24-28
Average 91 stars, based on 3 article reviews
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Knock-Out:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Expressing:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Cell Culture:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Immunodetection:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Modification:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), SDS Page:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Transfection:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Incubation:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Two Tailed Test:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Solubility:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Activation Assay:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), In Vivo:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Phospho-proteomics:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Staining:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Labeling:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), Hot Plate Test:Article Title: A Farnesyltransferase Inhibitor Restores Cognitive Deficits in<i>Tsc2</i><sup>+/–</sup>Mice through Inhibition of Rheb1 Article Snippet: HEK293 T cells, cultured hippocampal neurons, and mouse brains and livers were homogenized [40 mM Tris-HCl, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% Triton X-100, protease inhibitor cocktail tablet (Complete EDTA-free; catalog #11873580001, Roche) and 1 mM phenylmethanelsulfonyl fluoride (catalog #P7626, Sigma-Aldrich)] and centrifuged at 17,900 g at 4°C.and centrifuged at 17,900 g at 4°C. ... The resultant soluble and insoluble fractions were immunoblotted as described previously (Sugiura et al., 2015), and primary antibodies against FLAG (1:800; catalog #F3165, Sigma-Aldrich), |

